Respiration
Respiratory System:
- Primary function is to obtain oxygen for use by body's cells & eliminate carbon dioxide that cells produce
- Includes respiratory airways leading into (& out of) lungs plus the lungs themselves
- Pathway of air: nasal cavities (or oral cavity) > pharynx > trachea > primary bronchi (right & left) > secondary bronchi > tertiary bronchi > bronchioles > alveoli (site of gas exchange)
Respiratory system
www.niehs.nih.gov/oc/factsheets/ozone/ithurts.htm
Respiration
The exchange of gases (O2 & CO2) between the alveoli & the blood occurs by simple diffusion: O2 diffusing from the alveoli into the blood & CO2 from the blood into the alveoli. Diffusion requires a concentration gradient. So, the concentration (or pressure) of O2 in the alveoli must be kept at a higher level than in the blood & the concentration (or pressure) of CO2 in the alveoli must be kept at a lower lever than in the blood. We do this, of course, by breathing - continuously bringing fresh air (with lots of O2 & little CO2) into the lungs & the alveoli.
Breathing is an active process - requiring the contraction of skeletal muscles. The primary muscles of respiration include the external intercostal muscles (located between the ribs) and the diaphragm (a sheet of muscle located between the thoracic & abdominal cavities).
The external intercostals plus the diaphragm contract to bring about inspiration:
- Contraction of external intercostal muscles > elevation of ribs & sternum > increased front- to-back dimension of thoracic cavity > lowers air pressure in lungs > air moves into lungs
- Contraction of diaphragm > diaphragm moves downward > increases vertical dimension of thoracic cavity > lowers air pressure in lungs > air moves into lungs:
www.fda.gov/fdac/features/1999/emphside.html
Diaphragm
To exhale:
- relaxation of external intercostal muscles & diaphragm > return of diaphragm, ribs, & sternum to resting position > restores thoracic cavity to preinspiratory volume > increases pressure in lungs > air is exhaled
Intra-alveolar pressure during inspiration & expiration
As the external intercostals & diaphragm contract, the lungs expand. The expansion of the lungs causes the pressure in the lungs (and alveoli) to become slightly negative relative to atmospheric pressure. As a result, air moves from an area of higher pressure (the air) to an area of lower pressure (our lungs & alveoli). During expiration, the respiration muscles relax & lung volume descreases. This causes pressure in the lungs (and alveoli) to become slight positive relative to atmospheric pressure. As a result, air leaves the lungs (check this animation by McGraw-Hill).
Exchange of gases:
- External respiration:
- exchange of O2 & CO2 between external environment & the cells of the body
- efficient because alveoli and capillaries have very thin walls & are very abundant (your lungs have about 300 million alveoli with a total surface area of about 75 square meters)
- Internal respiration - intracellular use of O2 to make ATP
- occurs by simple diffusion along partial pressure gradients
- it's the individual pressure exerted independently by a particular gas within a mixture of gasses. The air we breath is a mixture of gasses: primarily nitrogen, oxygen, & carbon dioxide. So, the air you blow into a balloon creates pressure that causes the balloon to expand (& this pressure is generated as all the molecules of nitrogen, oxygen, & carbon dioxide move about & collide with the walls of the balloon). However, the total pressure generated by the air is due in part to nitrogen, in part to oxygen, & in part to carbon dioxide. That part of the total pressure generated by oxygen is the 'partial pressure' of oxygen, while that generated by carbon dioxide is the 'partial pressure' of carbon dioxide. A gas's partial pressure, therefore, is a measure of how much of that gas is present (e.g., in the blood or alveoli).
- the partial pressure exerted by each gas in a mixture equals the total pressure times the fractional composition of the gas in the mixture. So, given that total atmospheric pressure (at sea level) is about 760 mm Hg and, further, that air is about 21% oxygen, then the partial pressure of oxygen in the air is 0.21 times 760 mm Hg or 160 mm Hg.
Partial Pressures of O2 and CO2 in the body (normal, resting conditions): (check this animation by McGraw-Hill)
- Alveoli
- PO2 = 100 mm Hg
- PCO2 = 40 mm Hg
- Alveolar capillaries
- Entering the alveolar capillaries
- PO2 = 40 mm Hg (relatively low because this blood has just returned from the systemic circulation & has lost much of its oxygen)
- PCO2 = 45 mm Hg (relatively high because the blood returning from the systemic circulation has picked up carbon dioxide)
While in the alveolar capillaries, the diffusion of gasses occurs: oxygen diffuses from the alveoli into the blood & carbon dioxide from the blood into the alveoli.
- Leaving the alveolar capillaries
- PO2 = 100 mm Hg
- PCO2 = 40 mm Hg
- Entering the systemic capillaries
- PO2 = 100 mm Hg
- PCO2 = 40 mm Hg
- Body cells (resting conditions)
- PO2 = 40 mm Hg
- PCO2 = 45 mm Hg
- Leaving the systemic capillaries
- PO2 = 40 mm Hg
- PCO2 = 45 mm Hg
How are oxygen & carbon dioxide transported in the blood?
- Oxygen is carried in blood:
- 1 - bound to hemoglobin (98.5% of
all oxygen in the blood)
2 - dissolved in the plasma (1.5%)
Because almost all oxygen in the blood is transported by hemoglobin, the relationship between the concentration (partial pressure) of oxygen and hemoglobin saturation (the % of hemoglobin molecules carrying oxygen) is an important one.
Oxygen transport
Hemoglobin saturation:
- extent to which the hemoglobin in blood is combined with O2
- depends on PO2 of the blood:
The relationship between oxygen levels and hemoglobin saturation is indicated by the oxygen-hemoglobin dissociation (saturation) curve (in the graph above). You can see that at high partial pressures of O2 (above about 40 mm Hg), hemoglobin saturation remains rather high (typically about 75 - 80%). This rather flat section of the oxygen-hemoglobin dissociation curve is called the 'plateau.'
Recall that 40 mm Hg is the typical partial pressure of oxygen in the cells of the body. Examination of the oxygen-hemoglobin dissociation curve reveals that, under resting conditions, only about 20 - 25% of hemoglobin molecules give up oxygen in the systemic capillaries. This is significant (in other words, the 'plateau' is significant) because it means that you have a substantial reserve of oxygen. If you become more active, & your cells need more oxygen, the blood (hemoglobin molecules) has lots of oxygen to provide
When you do become more active, partial pressures of oxygen in your (active) cells may drop well below 40 mm Hg. A look at the oxygen-hemoglobin dissociation curve reveals that as oxygen levels decline, hemoglobin saturation also declines - and declines precipitously. This means that the blood (hemoglobin) 'unloads' lots of oxygen to active cells - cells that, of course, need more oxygen.
Factors that affect the Oxygen-Hemoglobin Dissociation Curve:
The oxygen-hemoglobin dissociation curve 'shifts' under certain conditions. These factors can cause such a shift:
- lower pH
- increased temperature
- increased levels of CO2
CO2 + H20 -----> H2CO3 -----> HCO3- + H+
& more hydrogen ions = a lower (more acidic) pH. So, in active tissues, there are higher levels of CO2, a lower pH, and higher temperatures. In addition, at lower PO2 levels, red blood cells increase production of a substance called 2,3-diphosphoglycerate. These changing conditions (more CO2, lower pH, higher temperature, & more 2,3-diphosphoglycerate) in active tissues cause an alteration in the structure of hemoglobin, which, in turn, causes hemoglobin to give up its oxygen. In other words, in active tissues, more hemoglobin molecules give up their oxygen. Another way of saying this is that the oxygen-hemoglobin dissociation curve 'shifts to the right' (as shown with the light blue curve in the graph below). This means that at a given partial pressure of oxygen, the percent saturation for hemoglobin with be lower. For example, in the graph below, extrapolate up to the 'normal' curve (green curve) from a PO2 of 40, then over, & the hemoglobin saturation is about 75%. Then, extrapolate up to the 'right-shifted' (light blue) curve from a PO2 of 40, then over, & the hemoglobin saturation is about 60%. So, a 'shift to the right' in the oxygen-hemoglobin dissociation curve (shown above) means that more oxygen is being released by hemoglobin - just what's needed by the cells in an active tissue!
Carbon dioxide - transported from the body cells back to the lungs as:
- 1 - bicarbonate (HCO3) - 60%
- formed when CO2 (released by cells making ATP) combines with H2O (due to the enzyme in red blood cells called carbonic anhydrase) as shown in the diagram below
- formed when CO2 combines with hemoglobin (hemoglobin molecules that have given up their oxygen)
Carbon dioxide transport
CO2 exchange in alveoli
Review questions:
What is the primary function of the respiratory system? What are the components of the conducting zone or division of the respiratory system? What makes up the respiratory zone or division? What muscles are involved in inspiration, expiration? What is caused by contraction of the inspiratory muscles, by relaxation of the inspiratory muscles? How & why does intra-alveolar pressure change during inspiration & expiration? What is external respiration & why is it so efficient? What is internal respiration? How does the exchange of gases occur in external respiration? What is partial pressure? What are the partial pressures of CO2 & O2 in the alveoli, alveolar capillaries (both coming in & going out), body cells, tissue capillaries (both going in & coming out)? How is oxygen transported in the blood? How does the partial pressure of oxygen affect hemoglobin saturation? What is the significance of the plateau in the oxygen-hemoglobin dissociation curve? What is the significance of the steep portion of the oxygen-hemoglobin dissociation curve? How do pH, temperature, 2,3-diphosphoglycerate, & CO2 affect hemoglobin molecules & the oxygen-hemoglobin dissociation curve? What is the significance of these effects? How is carbon dioxide transported in the blood?