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Growth, Nutrition, and Metabolism

Terms in this set (507)

A man succumbed to vibriosis due to a combination of swimming in the ocean before a tattoo was completely healed and an underlying condition of liver disease. In this case the tattoo was a ______ for the Vibrio vulnificus bacterium causing the infection.

portal of entry

The acquisition of chemical substances by organisms for the building blocks of cellular components and processes is

Nutrition

Which is the best term for any chemical substance that must be provided to a cell for normal metabolism and growth?

Nutrient

All living things have an absolute need for , such as carbon, hydrogen and oxygen.

bioelements

Any element or molecule that must be provided to an organism is called a(n) nutrient.

Essential

Incomplete healing of a tattoo led to a case of vibriosis because the tattoo site was a of for the Vibrio vulnificus bacterium.

portal, entry

______ is the acquisition of chemical substances by organisms for use as an energy source or as building blocks of cellular structures.

Nutrition

What term describes any chemical substance required in small quantities, such as a trace metal?

Micronutrient

Are essential chemical substances that must be consumed for normal cellular metabolism and growth

Nutrients

are chemical substances, such as sugars or amino acids, that are required in large quantities by cells.

macronutrients

Which of the following are bioelements?

oxygen
carbon
nitrogen
phosphorus
hydroge

Micronutrients (zinc, manganese) that are required by cells in small amounts are termed
elements.

trace

An essential nutrient is any element or compound that an organism

must get from a source outside its cells.

The function of micronutrients, or trace elements is(are):

maintenance of protein structure
proper enzyme function

Any chemical substance required in small quantities, such as a trace metal, is termed a(n)

Micronutrient

Molecules that contain a basic framework of carbon and hydrogen and are nearly always produced by living things are called ______ nutrients.

organic

Which term describes chemical substances such as carbon, hydrogen, and oxygen that are required in large quantities by cells?

Macronutrient

Living organisms obtain nutrients from ________ sources.

both organic and inorganic sources

Micronutrients such as zinc and manganese that are required by cells in small amounts are termed trace

Elements

Natural organic nutrients are usually the product of which of the following?

living organisms

Examples of _______ nutrients include magnesium sulfate, ferric nitrate, sodium phosphate, carbon dioxide, and water.

Inorganic

________ play an important role in enzyme function and protein structure.

Micronutrients

Molecules that contain a basic framework of carbon and hydrogen are called nutrients.

organic

Nutrient sources for all living organisms can be acquired from and compounds

organic; inorganic

Which of the following are the two most prevalent chemical components of a cell?

water
protein

molecules, which contain carbon, are nearly always the products of living organisms.

organic

are the organic compound found in the highest concentration in a cell.

Proteins

A(n) nutrient is composed of an element or elements other than carbon and hydrogen.

inorganic

Which of the following elements compose about 97% of a cell?

Sulfur
Nitrogen
Phosphorous
Oxygen
Hydrogen
Carbon

Molecules that contain a basic framework of carbon and hydrogen and are nearly always produced by living things are called ______ nutrients

organic

The element is represented by the letter N in CHONPS

Nitrogen

Which of the following is NOT true regarding the chemical composition of cells?

The most prevalent organic compound is nucleic acids.

Which of the following is the most prevalent organic compound found in cells?

Proteins

True or false: About 97% of the cell is composed of 6 elements.

True

Which element is represented by P in CHONPS?

Phosphorous

Which element is represented by N in CHONPS?

Nitrogen

The element is represented by the letter C in CHONPS.

Carbon

The element is represented by the letter S in CHONPS.

Sulfur

Which organism relies upon organic compounds for its carbon and energy needs?

Heterotroph

The element is represented by the letter P in CHONPS.

Phosphorus

Microbes that use photosynthesis to derive their energy are classified as ______, while microbes that gain energy from oxidizing chemical compounds are classified as ______.

phototrophs
chemotrophs

Which element is represented by C in CHONPS?

Carbon

Macronutrients in cells include carbohydrates, proteins, nucleic acids, and ______.

lipids

Which element is represented by S in CHONPS?

Sulfur

In addition to carbohydrates, proteins, and lipids, microbes can utilize as a source of essential nutrients.

nucleic acids

True or false: Proteins are organic macronutrients that can satisfy a heterotrophs need for carbon.

True

An organism which acquires energy by metabolizing the nutrients from other organisms is called a(n) ____________.

heterotroph

Which must acquire its chemical energy from some outside source?

Heterotroph

In addition to nucleic acids, proteins, and lipids, microbes require as a macronutrient source.

Carbohydrates

In addition to carbohydrates, proteins, and nucleic acids, microbes can utilize as a source of essential nutrients. (one word)

lipids

A(n) microbe requires only inorganic nutrients and carbon dioxide as a sole carbon source.

lithoautotrophic

Macronutrients in cells include carbohydrates, proteins, lipids, and ______ acids.

nucleic

bacteria lack the ability to synthesize some necessary organic compounds and will only grow if specific nutrients are provided.

fastidious

The majority of carbon compounds that a cell requires are:

Organic

A factor is an essential organic compound such as a vitamin or amino acid that must be provided in the diet

Growth

Which of the following receives their energy from the sun's light to generate a sugar source for cellular respiration?

Phototrophs

Macronutrients in cells include lipids, proteins, nucleic acids, and ______.

carbohydrates

Autotrophs can generate energy from which of the following?

Sun light
Simple, non-living chemical reactions

What term is used to describe organisms that can generate their own food from inorganic compounds?

Autotrophs

An autotrophic organism that utilizes light for energy and carbon dioxide primarily as a carbon source is specifically termed a

photoautotrophs

What is the term that describes bacteria that are unable to synthesize all of the organic compounds needed for survival?

fastidious

Photosynthesis that results in the production of oxygen is referred to as photosynthesis.

Oxygenic

What term is used to describe an essential organic compound such as a vitamin or amino acid that must be provided in the diet?

Growth factor

Photosynthesis that does not produce oxygen is known as ________ photosynthesis.

Anoxygenic

A -troph is an organism that uses the sun's light to provide the chemical energy to produce sugar for cellular respiration.

Photo

Microorganisms that use inorganic chemicals for energy and carbon dioxide for carbon are specifically termed

chemoautotroph

Autotrophs are special because

They can use fairly "free" energy sources to make sugar

Any microbe that uses photosynthesis to feed is specifically referred to as a(n) .

Phototroph

Which is a type of chemoautotroph that produces methane from hydrogen gas and carbon dioxide?

methanogens

_______ photosynthesis produces oxygen.

Oxygenic

What term is used to describe organisms which derive both energy and carbon from organic compounds?

Chemoheterotroph

Photosynthesis that does not utilize or produce oxygen is referred to as photosynthesis.

anoxygenic

Organisms that require organic compounds for their carbon and energy needs are known as:

heterotrophs

Which microorganism uses inorganic chemicals for energy and carbon dioxide for carbon?

Chemoautotrophs

The principal energy-yielding pathway that involves the breakdown of glucose in the presence of oxygen is called .

Aerobic respiration

Methanogens are

chemoorganic autotrophs
archaea

A -troph derives both energy and carbon from organic compounds.

chemohetero

A heterotroph that decomposes organic remains from dead organisms is also called a(n) .

saprobe

Organic compounds are the carbon and energy source for heterotrophs.

True

When a normally nonpathogenic saprobe causes infection in a compromised host, it is referred to as a(n) pathogen.

Opportunistic

Microorganisms that use inorganic chemicals for energy and carbon dioxide for carbon are specifically termed .

chemoautotroph

_______ involves the complete breakdown of glucose to yield carbon dioxide, water, and energy in the form of ATP.

Aerobic respiration

Organisms that derive nutrients from the living hosts are called _______.

parasite

Any microorganism that causes disease is a(n) _______.

pathogen

What term is used to describe organisms that decompose organic remains from dead organisms as an energy source?

Saprobe

An organism that is unable to grow outside of a living host is referred to as a(n) intracellular parasite.

Obligate

______ pathogens are usually nonpathogenic microbes that cause disease in a compromised host.

Opportunistic

Which of the following cell structures is specialized for transport of materials into and out of the cell?

Cell membrane

A is any organism that obtains its nutrients from the cells or tissues by taking them from another living host.

Parasite

The speed and continuous movement of atoms and molecules is largely controlled by ________.

temperature

A is any microorganism that causes disease.

pathogen

Which of the following is the movement of molecules along a concentration gradient by spontaneous random motion to achieve a uniform distribution?

diffusion

A(n) _________ is unable to grow outside of a living host.

obligate parasite

is the diffusion of water across a selectively permeable membrane in the direction of lower water concentration.

Osmosis

A(n) permeable membrane can allow specific compounds into the cell and/or out of the cell.

selectively

The cell is specialized to transport substances into and out of the cell.

membrane

Which of the following would describe a solution which is well balanced for a cell?

Isotonic

True or false: All atoms and molecules are in a constant state of movement that increases with temperature increases.

True

The movement of molecules along a concentration gradient by spontaneous random motion to achieve a uniform distribution is termed .

diffusion

A solution which is will cause a cell to swell with water.

hypotonic

Which term is used exclusively to describe water's movement across a selectively permeable membrane?

Osmosis

Which type of solution contains a higher concentration of solutes?

Hypertonic

Membranes that only allow certain compounds into or out of the cell are called ______.

Selective

-lysis occurs to a bacterial cell when it loses water due to an environment that has far more solutes outside the cell compared to inside.

Plasmo

A solution in which the concentration of solutes is the same on both sides of a membrane is .

Isotonic

Organisms with cell walls become _______ in a hypotonic solution because the cell wall prevents them from bursting.

turgid

The cell controls diffusion of most substances into and out of the cell.

Membrane

If a cell is in a solution that is hypotonic relative to the cytoplasm, how will water move?

Into the cell by osmosis

During facilitated diffusion, molecules are transported across the membrane with the help of carriers

proteins

If a cell loses water after being placed into a particular solution, what is true regarding the osmolarity of the solution?

The solution is hypertonic relative to the cell.

True or false: Each different carrier protein can only bind and transport a certain molecule.

True

Bacterial cells placed in a solution where the concentration of solute within the cytoplasm is less than that of the extracellular solution would undergo ______.

Plasmolysis

In transport, the cell supplies energy for compounds to move into or out of the cell.

active

A cell that has become swollen due to the influx of water is referred to as

Turgid

The diffusion of materials into a cell is largely controlled by which structure?

cell membrane

ATP is required in - transport used to transport certain sugars, amino acids, vitamins, and phosphate into the cell.

Carrier
Mediated
Active

Which of the following would move across the cell membrane by facilitated diffusion?

glucose

In the specific process of , active transport is achieved by coupling the transport of a nutrient with its conversion to a substance that is immediately useful to the cell.

group
translocation

What term is used to describe the "best" temperature to support a microbe's growth?
Multiple choice question.

Optimal temperature

Carrier proteins exhibit , which means they can only transport certain molecules.

specificity

White blood cells and amoebas transport large particles and other cells through .

phagocytosis

Which of the following is a cellular transport system that requires cellular energy?

Active transport

Gradual adjustment and evolutionary change that allows microbes to survive in their environment is known as _______.

adaptation

The range of temperatures for microbial growth is expressed as three temperatures.

ardinal

_______ active transport requires ATP to transport specific sugars, amino acids, and vitamins into the cell.

Carrier-mediated

The temperature is the highest temperature at which an organism will grow.

maximum

When a molecule is moved across a membrane and is simultaneously converted into a metabolically active substance, ________ has occurred.

group translocation

is the act of successfully adjusting to a new environment.

Adaptation

Which term describes a microorganism that thrives and divides under refrigeration conditions?

psychorphile

Which of the following is a NOT a cardinal temperature related to microbial growth?

Niche temperature

Microbes that can grow slowly in cold temperatures, but have optimum temperatures above 20°C, are called ______.

Psychrotrophs

The temperature is the lowest temperature at which an organism will grow.

minimum

Because mesophilic bacteria have an optimal temperature of ° Celsius (same as human body temperature), they are the largest group of medically important microorganisms.

37

The temperature at which a species shows the most rapid growth rate is known as the cell's temperature.

Optimal

Microorganisms that are normally mesophiles, but can handle short exposures to high temperatures are called microbes.

thermoduric

From which environment would you most likely observe growth of psychrophilic microorganisms?

Frozen food

Where would a thermophilic microorganism be found?

In natural hot springs

_______ can grow in the cold and are able to cause food-borne illness, but are not true psychrophiles.

Psychrotrophs
Facultative psychrophiles

Choose the temperature range of most mesophilic microorganisms.

20°C-40°C

Which type of microbes are a common contaminant of pasteurized or heated foods?

Thermoduric microbes

A is a microorganism, such as an archaean, that thrives at extremely high temperatures (above 80°C-110°C).

Hyperthermophiles

Microorganisms that prefer hot environments in the range of 45°C to 80°C are called -philes.

thermo

The two atmospheric gases that have the greatest impact on microbial growth are and .

oxygen
carbon
dioxide

Hydrogen peroxide is converted into water and oxygen by the enzyme _______.
Multiple choice question.

catalase

Based upon the descriptions, identify the tube growing the obligate anaerobe

tube with heavy growth toward the bottom

Microorganisms which prefer extremely hot environments (80°C-110°C) are called ___

hyperthermophile

What word describes a microbe that must have oxygen to survive?

Obligate aerobe

The atmospheric gases that most influence microbial growth are:

Oxygen
Carbon dioxide

Which is FALSE regarding a facultative anaerobe?

The electron transport system is non-functional

The enzyme converts hydrogen peroxide formed during aerobic respiration to water and oxygen.

catalase

What word is used to describe organisms which do NOT use oxygen in their cellular respiration pathways?

Anaerobic

An is a microorganism that can use oxygen in metabolism and process toxic oxygen products.

aerobe or aerobic

A(n) aerobe, or strictly aerobic organism, requires oxygen for survival.

Obligate

A strict or anaerobic microorganism requires the absence of oxygen for survival.

Obligate

A sealed jar filled with nitrogen gas or carbon dioxide can be used to cultivate bacteria.

anaerobic

A(n) anaerobe is capable of growing in aerobic and anaerobic conditions.

Facultative

____________ _________ do not use oxygen gas but are not affected by its presence.

Aerotolerant anaerobes

A(n) is any microorganism that grows best in the absence of oxygen. (one word)

anaerobe or anaerobic

What word is used to describe organisms which can use oxygen in their cellular respiration pathways?

Tube with heavy growth toward the bottom

A(n) is an organism that prefers carbon dioxide levels higher than those present in the normal atmosphere.

Capnophile

A sealed jar filled with nitrogen gas can be used to cultivate

anaerobes

An organism that does not use oxygen gas, but can survive and grow in its presence is referred to as a(n) .

Aerotolerant anaerobe

are organisms that thrive in environments with a pH close to 7.

Neutrophiles

A(n) is a microorganism that thrives in any hypertonic medium.

Osmophile

Which term describes bacteria that require or tolerate high concentrations of salt?

Halophile

An organism that requires carbon dioxide levels higher than those present in the normal atmosphere is called a ______.

capnophile

are organisms which thrive in the pressure of deep ocean depths.

barophiles

Which term describes any close relationship between two organisms?

Symbiosis

What term is used to describe organisms that exist in environments near the pH of 7?

Neutrophiles

Which term describes a microbe that grows in a habitat with a high solute concentration?

Osmophile

Which of the following describes a type of symbiotic relationship between two organisms in which both species benefit?

Mutualism

A(n) specifically requires a high concentration of salt for growth.

halophile

is the process by which a change in one symbiotic partner leads to a change in the other, which may in turn lead to change in the first partner, and these changes can continue to occur over time.

coevolution

What term is used to describe organisms that exist at deep ocean depths?

Barophiles

is the term used to describe a situation where organisms gain mutual benefit from their association but can survive independently outside the partnership.

Cooperation

is a close association between individuals from two species that may be helpful, harmful or neither to both members.

Symbiosis, Symbiotic, or Symbioses

is a type of symbiotic relationship between two organisms in which one species derives benefit without harming the other.

commensalism

is a type of symbiotic relationship between two organisms in which both species benefit.

Mutualism

A symbiotic relationship where one organisms benefits at the expense of another is termed ____.

parasitism

A genetic or evolutionary change in one symbiotic partner that leads to a genetic or evolutionary change in the other symbiotic partner is known as ________.

coevolution

A situation where a microbe spends all or most of its life cycle inside a host cell is called parasitism.

obligate, intracellular

A nondependent form of mutualism, where both partners can survive independently outside the relationship is called ______.

cooperation

Which relationships do not require a close association between microbes?

amensalism
syntrophy

Which of the following is defined as a relationship between organisms where one benefits and the other is unharmed?

Commensalism

A beneficial relationship involving communal feeding between organisms sharing a habitat is called .

Syntrophy

is a relationship between two organisms where the host is harmed and the colonizer benefits.

Parasitism or Parasitic

An association where a parasite spends all or most of its life cycle gaining nutrients and support within a host cell is called ______ parasitism.

obligate intracellular

Which of the following describes a relationship where microorganisms compete for survival in a common environment?

Amensalism

Which relationship does not require a particularly close association between microbes or between microbes and other organisms?

Amensalism

Antibiotics released by one microbe to inhibit or kill other microbes demonstrates an antagonistic relationship called .

Amensalism

In one form of amensalism, microbes release specific chemicals called ______ into the environment to inhibit or kill other microbes.

Antibiotics

A relationship in which the products given off by one organism are usable as nutrients for another organism is called ______.

syntrophy

A is a complex association of microorganisms in which the members are bound to each other and a substrate by an extracellular matrix.

Biofilm

A relationship where microorganisms compete for survival in a common environment is known as .

antagonism

True or false: Biofilms are very common.

True

The normal resident bacteria found in or on the human body are collectively called the ______.

microbiota

When organisms attach to a substrate and secrete an extracellular matrix, a _______ is formed.

biofilm

sensing is the ability of some microbes to detect the number of other microbes and act accordingly.

Quorum

Most microbes on earth live in a complex, organized association known as a(n) _________.

biofilm

Molecules released by members of the biofilm that accumulate as the population grows and stimulate gene expression in its members are called molecules.

inducer

A(n) _______ is a critical number of cells required to release inducer molecules in order to form a biofilm.

quorum

The bacteria that normally inhabit the skin, GI tract, and other body sites are also referred to as normal resident

Flora

Bacteria reproduce by fission.

binary

Which terminology describes the ability of some microbes to detect the numbers of other microbes and alter their behavior?

Quorum sensing

In binary fission, the doubling time of a particular microbial species is also known as its time.

generation

In a normal bacterial growth curve, what quantity is represented on the x-axis?

Time

What are the compounds that are released by members of a biofilm that accumulates as the population grows and stimulate gene expression in its members?

Inducer molecules

Escherichia coli has a generation time of 20 minutes. If 1 million parent cells were placed in a flask of sterile nutrient broth and incubated under optimal growth conditions, approximately how many cells would be present in the broth after 1 hour?

8 million

A critical number of members required in a group, such as a minimum number of cells in a population, is referred to as a(n) .

quorum

After a bacterial culture acclimates to a medium, the bacteria will divide in what type of pattern?

Exponential

Which is FALSE regarding binary fission?

It leads to genetic variation.

Which term describes the length of time of a microbial species needs to divide?
Multiple choice question.

Generation time

What term is used to describe a graphical representation of a change in population size over time?

Growth curve

True or false: The rate of growth in a bacterial culture can be measured in hours, days, or weeks depending upon the species.

True

What common term describes the single bacterium in an original sample that gives rise to a colony on a plate?

Colony forming unit

Cell population growth can be represented by the number ______ with an exponent, where the exponent increases by ______ in each generation.
Multiple choice question.

2; 1

The correct order for the phases of a normal population growth curve is

lag, log, stationary, death

Which set of numbers describe bacterial growth after the culture has acclimated to the medium's conditions?

1, 2, 4, 8, 16, 32, 64

phase is when a bacterial cell is placed into a new environment and cell division is slow.

Lag

Limited growth during the lag phase of a growth curve is due to the need for cells to

...

During the phase of microbial growth, numbers of cells are difficult to count because cells are adjusting to growth media, they have not reached their maximum growth rate, and the overall number of cells is sparse.

lag

A curve is a graphical representation of the change in population size over time.

growth

The log phase of a normal growth curve is also referred to as the growth phase.

exponential

unit is a term used to describe a single bacterium that gave raise to an entire colony.

Colony
forming

phase of bacterial cell growth is when the number of cells dividing equals the number of cells that are dying.

stationary

Put the phases of a normal population growth curve in order.

Lag phase
Log phase
Stationary phase
Death phase

Which of the following characterizes the lag phase of a normal bacterial growth curve?

Little increase in cell numbers

Limited growth during the lag phase of a growth curve is due to the need for cells to
Multiple choice question.

synthesize new enzymes to metabolize nutrients in the culture media.

In which phase of bacterial growth are far more cells dividing than dying?
Multiple choice question.

Exponential

During which phase of growth does the number of new cells balance the number of declining cells?

Stationary phase

Which phase of bacterial growth involves more cells dying than reproducing?

Death

What term is used to describe a continuous culture system that provides nutrients and removes waste to stabilize cell cultures?

Chemostat

The term describes the appearance of a cloudy broth culture.

turbid or turbidity

Enumeration of bacteria can be achieved by the cell count, in which a cell suspension in a chamber is viewed under the microscope.

direct

The phase of bacterial cell growth is when the number of cells dividing equals the number of cells that are dying.

Stationary

True or false: A Coulter counter can differentiate between living and dead or gram-positive and gram-negative organisms.

False

The phase of the population growth curve involves very little cell division and an exponential decline in numbers.

Death

A is a continuous culture system that provides nutrients and removes waste to stabilize the growth rate and cell number of cultures.

chemostat

Identify the ways that salt and/or sugar inhibit microbial growth.

-Increasing osmotic pressure
-Reducing the water activity needed for survival and growth
-Conversion to compounds with anti-microbial activity
-Weakening the molecular structure of DNA

What term is used to describe the cloudiness of a broth culture?

Turbidity

The count is obtained using a procedure that involves counting the number of cells in a sample microscopically using a special calibrated slide with a premeasured grid.

Direct cell

A high level of blood alcohol caused by reactions in the small intestine that use carbohydrates to produce alcohol causes ______ syndrome.

gut fermentation

A electronically scans a culture as it passes through a tiny pipette in order to obtain a cell count, but cannot differentiate between living and dead cells..

Coulter
counter

Which phase of bacterial growth involves more cells dying than reproducing?
Multiple choice question.

Death

The combination of all reactions (catabolic + anabolic) within a cell is termed .

metabolism or metaballein

Salt or sugar can be used to increase ______ that will restrict the growth of bacteria in foods.
Multiple choice question.

osmotic pressure

refers to the decomposition of complex compounds during cellular metabolism, usually with the release of energy.

Catabolism

In very rare instances of intoxication, yeast in the small intestine can convert dietary carbohydrates to alcohol, a situation called gut syndrome.

fermentation

A(n) is a biological catalyst.

enzyme

The term "metabolism" includes which type(s) of cellular reactions?

Both anabolic and catabolic

Most enzymes are a functional type of (a type of macromolecule).

protein

Which of the following is the decomposition of complex compounds during cellular metabolism?

Catabolism

Which of the following macromolecules can commonly act as a catalyst?

Proteins

Composed principally of amino acids, ______ act as biological catalysts, facilitating metabolic activities of the cell.

enzymes

Which statement is FALSE regarding enzymes?

Create reactions

The minimum amount of energy required for a reaction to proceed is termed the energy of .

activation

Which best describes the function of enzymes?

Facilitate metabolic pathways

Which is NOT a method of overcoming activation energy in any reaction?

Decreasing the substrate concentration

An enzyme acts as a biological that alters the rate of a reaction without being changed by the reaction.

catalyst

Enzymes are indispensable for life because they

can speed up the rate of an uncatalyzed reaction by up to 100 trillion fold.

What term is used to describe the minimum amount of energy required for a reaction to proceed?

Activation energy

Enzymes typically lower the energy needed for a reaction to proceed.

activation

The is the term for a specific molecule on which an enzyme acts.

substrate

Identify any of the following which could INCREASE the rate of an enzymatic reaction which is currently not at optimal conditions.

-Decrease temperature slightly
-Increase temperature slightly
-Increase substrate(s) concentration
-Increase enzyme concentration

Typically, the presence of enzymes ____ the activation energy for a reaction to proceed.

lowers

What name is given to the compound upon which an enzyme will act?
Multiple choice question.

Substrate

Enzymes are typically ____ than substrates.

larger

Typically, once an enzyme performs a "job", it is

ready to "work" again

A non-functional enzyme which needs a cofactor is called a(n) .

apoenzyme

A(n) enzyme consists of protein alone, while a(n) enzyme (or holoenzyme) consists of protein and nonprotein components.

simple
conjugated

Substrates are generally than the enzymes to which they bind.

smaller

For an apoenzyme to become a functional holoenzyme, it must ______.

bind to a cofactor

Which of the following is needed for an apoenzyme to be fully functional?

Cofactor

Which of the following statements about enzymes are true?

-Enzymes bind their substrate but are not changed in the reaction.
-Enzymes lower the energy of activation for the reaction that they catalyze.
-Enzymes are not consumed in the reaction they catalyze.

What term refers to a biological catalyst that lacks an essential cofactor?
Multiple choice question.

Apoenzyme

Which of the following represent cofactors?

Metal ions
Coenzymes
Small organic molecules

An enzyme with another molecule, such as a metal or vitamin cofactor, bound covalently to it is termed a(n) _______.

conjugated enzyme

A catalyst composed of RNA is called a(n) .

Ribozymes

An enzyme with its apoenzyme and cofactors is referred to as a(n) , or a conjugated enzyme.

Haloenzyme

Protein folding determines the 3-D shape in enzymes needed for their for a particular substrate.

specificity

A(n) is an organic molecule or inorganic element that may be required for an enzyme to become functional.

cofactor

Which best defines an active site?

Region of an enzyme where the substrate binds

Which of the following could not be a cofactor?

Organic acids

Besides proteins, which type of molecule has been shown to have catalytic activity?

RNA

Which aspect of an enzyme's structure gives rise to the specificity for its substrate?

Folding to produce a 3-D structure

Which feature of an enzyme is the "most" unique?

Active site

The site is the region on an enzyme that binds substrate.

active

Enzyme-substrate interactions are sometimes referred to as a(n) " and key" interaction.

lock

Slight changes occur in the shape of the enzyme in order to guide the substrate into the active site. This is referred to as a(n) .

induced
fit

The bonds that form enzyme-substrate complexes are and easily reversible.

weak

An enzyme's site is unique for each substrate.

active

Often derived from vitamins, a ______ is a type of cofactor that will act in conjunction with an enzyme to carry out activity.

coenzyme

When combined with its apoenzyme, a(n) is an organic compound that functions to move (carry) functional groups from one substrate to another.

coenzyme

Which model is sometimes used to describe enzyme-substrate interactions?

Lock-and-key

When a substrate binds to an enzyme's active site, often an _____ occurs which brings the substrate tightly within the active site pocket.

induced fit

Enzymes bond _____ with their substrate.

weakly

A complex organic molecule, often derived from vitamins, that acts in conjunction with an enzyme is known as a(n) .

Coenzyme

are principle components of coenzymes and this is why they are important components of our diet.

Vitamins

Which of the following acts as a carrier to move a chemical group from one substrate molecule to another?

Coenzyme

A(n) describes any enzyme that works outside a cell in which it was produced.

exoenzyme

A(n) describes any enzyme that works within a cell in which it was produced.

Exoenzyme

A(n) enzyme describes an enzyme present in constant amounts regardless of the presence of substrate.

Constitutive

Which of the following is the most common component of coenzymes?
Multiple choice question.

Vitamins

A(n) enzyme describes an enzyme that is not always present in the cell since its production responds to changes in concentration of substrate or product.

regulated, inducible, or repressible

What term is used to describe enzymes which work outside the cell?

Exoenzymes

What term is used to describe enzymes which work within the cell that produces them?

Endoenzymes

Most condensation reactions require an input of and result in a loss of .

energy
water

What name is used to describe enzymes that are generally found at constant amounts in the cell independent of substrate concentrations?

Constitutive enzymes

Reactions that involve the breaking of bonds and an input of water are called ______ reactions.

Hydrolysis

Which is NOT a physical condition needed for optimal function of an enzyme?

Substrate concentration

The regulated enzymes

is produced in response to changes in substrate or product concentration.

Most condensation reactions involve the loss of:

water

Labile means which of the following?

Chemically unstable

The digestion of larger substrates into smaller molecules, involving the use of water, is usually the result of reactions.

Hydrolysis

The loss of the normal or native shape of a protein due to chemical or physical disruption of tertiary structure is called protein .

Denaturation

Which of the following can result in the denaturation of enzymes?

Chemicals
High temperatures
Changes in pH

A(n) enzyme describes an If internal cellular enzymes are denatured, it can lead to:

cell death

Typically, each reaction (step) in a metabolic pathway will require

one enzyme

Compounds are called when they can be easily denatured, distorted and/or degraded by mild changes to the environment.

labile

One evolutionary strategy to deal with an "enzymatic-block" to a particularly necessary metabolic product is to

have alternative enzymatic methods to synthesize the product

What term is used to describe proteins losing their three dimensional structure as a consequence of changes in the environmental conditions such as excessive heat or salt?

Denaturation

The product of one reaction may act as the for the next reaction in a metabolic pathway.

...

Each reaction in a metabolic pathway is catalyzed by a separate .

enzyme

Often, regulation of metabolic pathways occurs with activation or repression of the -maker enzyme.

pace

True or false: Metabolic pathways are interconnected.

True

In inhibition, a molecule occupies the active site which prohibits the substrate from binding and therefore, inhibits the enzyme's activity on the substrate.

competative

In metabolic pathways, the products of one enzymatic reaction may act as the ________ for the next step in the pathway.

...

In metabolic pathways, the products of one enzymatic reaction may act as the ________ for the next step in the pathway.

Substrate

Metabolic pathways in which the starting molecule is regenerated through the series of reactions are called ______ pathways.

cyclic

What is the most common target when cells regulate metabolic pathways?

Pacemaker enzyme

Competitive inhibition decreases the activity of an enzyme by

Blocking the active site of the enzyme
Preventing the enzyme-substrate interaction

Inhibitors that share binding affinity with the substrate is termed a ______ inhibitor.

competitive

The regulatory site of an enzyme can also be referred to as the site.

allosteric

Most competitive inhibitors found in cells are

products of metabolic pathways

When the starting molecule of a biochemical pathway is regenerated through the series of connected reactions, it is referred to as a(n) pathway.

cyclic

Negative feedback is a process that ______ enzyme activity when a certain concentration of ______ is produced.

decreases; product

An inhibitor which binds to a site other than the active site and controls enzyme activity is called a ____ inhibitor.

noncompetitive

The terminology "enzyme repression" implies that the enzyme is

not longer being synthesized

The activity of an enzyme can be controlled through the binding of molecules to a site other than the active site that is called a(n) ______.

regulatory site

All cells need a constant input and expenditure of ________ in a usable form.
Multiple choice question.

energy

In inhibition, the regulator is often a product of the enzymatic reaction.

competitive

Select the ways that cells manage the energy needed for metabolic reactions.

-Transferring electrons
-Making and breaking chemical bonds

A mechanism that can slow down enzymatic activity once a certain concentration of product is produced is called feedback.

negative

In enzyme inhibition, a regulatory molecule binds to a site other than the active site.

noncompetitive

The term enzyme refers to an inhibition of the genetic apparatus responsible for replacing the enzyme which results in a decrease in the number of enzyme molecules.

Repression

All living cells require a constant input and expenditure of usable .

Energy

Chemical reactions that release energy as they proceed are called reactions.

exergonic

Energy management in a cell most often involves the making or breaking of chemical and the transfer of from one molecule to another.

Bonds, electrons

Chemical reactions that are driven forward with the addition of energy are called reactions.

endergonic

A chemical reaction that releases energy as it proceeds is referred to as a(n) ______ reaction.

exergonic

Paired reactions where an electron donor transfers electrons to an electron acceptor are called ______ reactions.

redox

Compounds that are oxidized will

lose electrons

A redox reaction involves the coupling of which two types of reactions?

Oxidation and reduction

A chemical reaction that requires energy to proceed is referred to as a(n) ______ reaction.

endergonic

The addition of a phosphate group is referred to as .

phosphorylation

Collective reactions which transfer hydrogens/electrons from one compound to another are termed reactions.

Redox

Using an abbreviated acronym, name any one of the common coenzymes acting as electron carriers in your cells: (Do not add the hydrogen to the end)

FAD

Oxidation reactions are coupled with reactions.

reduction

Which of the following couples (links) anabolic reactions with catabolic reactions?

ATP

In a redox reaction, one compound is and another compound is .

REDUCES, OXIDIZED

Which nitrogenous base is found in ATP?

Adenine

How many phosphate groups does ATP contain?

3

Which term is used to describe the addition of a phosphate group?

Phosphorylation

Which is NOT a common cellular coenzyme that carries electrons?

ADP

Which of the following sugars is part of the structure of ATP?

Ribose

is the energy currency of the cells, coupling energy releasing reactions with energy requiring reactions.

ATP

The nitrogenous base is a component of ATP.

adenine

The high energy of ATP is located in the bonds of the groups.

phosphate

ATP has phosphate groups. (number)

3

To form ADP, a total of phosphate bond(s) must be broken from ATP.

1

Which of the following is best associated with the electron transport system?

Oxidative phosphorylation

ATP contains an adenine linked to , a 5-carbon sugar.

ribose

Photosynthetic organisms use a sunlight-driven electron transport chain to generate ATP via ________.

photophosphorylation

The study of the mechanisms of cellular energy release, including catabolic and anabolic pathways, is called .

Bioenergetics

Where do you find the high energy bonds in a molecule of ATP?

The last phosphate groups

How many bonds must be broken in ADP to form AMP?

1

The three coupled pathways that many organisms utilize during the catabolism of fuel molecules are , the cycle, and the chain.

Krebs cycle, glycolysis, respiratory chain

An electron transport system and chemiosmosis are used to produce ATP in non photosynthetic microorganisms via phosphorylation.

oxidative

Which of the following is correct regarding aerobic and anaerobic respiration pathways?

Aerobic pathways use oxygen.

Photosynthetic microorganisms produce ATP via using an electron transport system powered by light and water.

photophosphorylation

In respiration, electrons are transferred from fuel molecules to oxygen which acts as the terminal electron acceptor.

Aerobic

Which term is used to describe the study of the mechanisms of cellular energy release, including catabolic and anabolic pathways?

Bioenergetics

Which is the primary pathway used by heterotrophic microorganisms to obtain energy?

Aerobic respiration

During the last steps of glycolysis, ATP is generated by phosphorylation

substrate
level

The primary catabolism of molecules by most organisms would include which three of the following pathways?

Kreb's cycle
Respiratory chain
Glycolysis

Which of the following compounds make the "best" electron donors in aerobic respiration?

Sugars

The major difference between aerobic and anaerobic respiration is ______.

using or not using oxygen

The term "aerobic respiration" implies that ____ is required for the metabolic pathway to be completed.

oxygen

Identify any of the following which serve as energy-poor products of aerobic respiration.

Water
CO2

Most heterotrophic organisms use respiration to obtain energy.

aerobic

What occurs in the last 3 steps of glycolysis?

ATP forms by substrate-level phosphorylation

are used as cellular energy sources because they are superior electron donors.

Carbohydrates

Which of the following is an energy-poor product of aerobic respiration?
Multiple choice question.

Both CO2 and water

Which is an energy-rich product of aerobic respiration?

ATP

Which of the following metabolic pathways begins with glucose and yields two pyruvates?

Glycolysis

Glycolysis has enzymatic steps for glucose to be converted to two pyruvates. (number)

9

The first step in glycolysis is the of glucose to produce glucose-6-phosphate.

phosphorylation

(acronym) is the useful final product of aerobic respiration.

ATP

Glucose-6-phosphate is converted into -6-phosphate in the second reaction of glycolysis.

fructose

is the multi-step energy-yielding conversion of glucose to pyruvic acid.

Glycolysis

Why do the last five reactions of glycolysis occur twice for each glucose substrate?

Glucose is split into 2 intermediates at step 4

Glycolysis consists of how many steps?

9

Glycolysis provides a net gain of two ATP molecules through ______ phosphorylation.

substrate-level

What is the first step in glycolysis?

Phosphorylation of glucose

By the end of glycolysis, glucose is converted into two 3-carbon molecules.

pyruvic
acid

What happens to glucose-6-phosphate in glycolysis?

Converted into fructose-6-phosphate

For each molecule of glucose that enters glycolysis, the last five reactions (of glycolysis) occur times.

2

The NET output of glycolysis is ATP, NADH, and pyruvic acid molecules per glucose.

2
2
2

During glycolysis, ATP is made directly by - phosphorylation.

substrate
level

In strict aerobes and some anaerobes, pyruvic acid enters the , or citric acid, cycle.

Krebs

enzyme inhibition is a mechanism of regulation where the active site is blocked by a molecule that resembles the true substrate.

competitive

At the end of glycolysis, the carbons from glucose are now located in

two pyruvic acids.

The location of the Krebs cycle enzymes in eukaryotes is in the matrix, while in prokaryotes the enzymes are found in the .

mitochondrial cytosol

For each glucose that enters glycolysis, the NET output is:

2 NADH
2 Pyruvic acid
2 ATP

Glycolysis provides a net gain of two ATP molecules through ______ phosphorylation.
Multiple choice question.

substrate-level

What is the typical "next step" for pyruvate at the end of glycolysis following normal aerobic or anaerobic respiration?

Krebs cycle

The atom is added to NAD to reduce it.

Hydrogen

The Krebs cycle occurs in the ______ of eukaryotic cells and the ______ of bacteria.
Multiple choice question.

mitochondrion; cytoplasm

For each glucose that enters glycolysis, acetyl CoA formation results in the production of ______.

2 CO2
2 NADH

-CoA feeds directly into the Krebs cycle.

acetyl

During the conversion of pyruvic acid to acetyl CoA, electrons reduce .

NAD

At the end of glycolysis, the carbons from glucose are now located in
Multiple choice question.

two pyruvic acids.

The Kreb's cycle has steps.

8

During acetyl CoA formation CO2 and NADH are produced per initial glucose that enters glycolysis.

2
2

For each glucose that enters glycolysis, the Kreb's cycle produces:

2 ATP
4 CO2
6 NADH
2 FADH2

The first step of the Krebs cycle results in the formation of ________.

citric acid

The ATP generated in the Krebs cycle is produced by _________ phosphorylation.

substrate level

How many steps compose the Krebs cycle?

8

In the last step of the Krebs cycle, is dehydrogenated to produce which is then available to react with acetyl-CoA to start the cycle again.

malate
oxaloacetate

In the last step of the ETS, the electrons are passed to along with hydrogen which results in the formation of .

oxygen or O2
water or H2O

During the Kreb's cycle CO2, FADH2, NADH, and ATP are produced per initial glucose that enters glycolysis.

4 or four
2 or two
6 or six
2 or two

The first step of the Krebs cycle is the formation of acid from acid and the acetyl group of acetyl CoA.

citric
oxaloacetic

The Krebs cycle generates ATP by phosphorylation.

substrate
level

Which Krebs cycle substrate is converted to oxaloacetate so that the cycle can begin again?

Malate

The final step of the ETS in aerobic respiration occurs when electrons are passed to ________.

O2

What compound is composed of a heme (iron containing) component which can shuttle electrons through the ETS during respiration?

Cytochrome

Which component of the electron transport chain accepts electrons from NADH?

Complex I

Complex II of of the electron transport chain accepts electrons from the carrier called .

FADH2

A(n) is an iron containing (heme) protein electron carrier in the last phases of aerobic respiration.

Cytochrome

Coenzyme Q is also known as , because it is very common in cells.

Ubiquinone

ATP synthase is principally composed of ______.

amino acids

What term is used to describe the process of ATP production from the ETS?

Oxidative phosphorylation

Complex I in the electron transport chain accepts electrons from the carrier called .

NADH

Which group of iron-sulfur proteins in the electron transport chain accepts electrons from FADH2?

Complex II

Another name for Coenzyme Q is

Ubiquinone

Production of ATP occurs by the process of ______, where hydrogen ions travel down their concentration gradient through channels in ATP synthase complexes.

chemiosmosis

ATP is an enzyme in the mitochondrial cristae that harnesses the flux of hydrogen ions across the membrane during oxidative phosphorylation.

synthase

The active pumping of hydrogen ions across the cristae membrane sets up a concentration gradient of hydrogen ions called the .

Proton motive force (PMF)

ATP is synthesized via phosphorylation during the electron transport phase of respiration.

Oxidative

If the membranes of the mitochondrion were permeable to protons then what would happen?

Loss of proton motive force

The charged gradient which is formed in the ETS results in the

proton motive force

In eukaryotes, ATP synthase enzymes are found

on the inner membrane of the mitochondria

is the generation of a proton motive force by the pumping of hydrogen ions to the outer side of the membrane during electron transport and the movement of those hydrogen ions down their electrochemical gradient through the ATP synthase enzyme.

Chemiosmosis

In bacteria, protons are moved from the cytoplasm to the space during chemiosmosis.

Periplasmic

As hydrogen ions accumulate between the inner and outer membranes of the mitochondrion, a(n) _____ is generated which powers ATP production.

proton motive force

The electron transport chain is located in the ______ membrane of eukaryotic cells and the ______ membrane of bacteria.

inner mitochondrial; plasma

The impermeability of the inner cristae membrane to is crucial for the maintenance of the chemiosmotic gradient.

protons

In eukaryotic cellular respiration, the charge gradient that exists between the inner and outer membranes during chemiosmosis is called proton motive force.

Mitochondrial

Which component of the electron transport system catalyzes the terminal step of aerobic respiration?

Cytochromes a and a3

Protons can only cross the mitochondrial inner membrane through .

ATP synthase

Cyanide causes rapid death in humans and other eukaryotes because it terminates aerobic respiration by blocking cytochrome .

oxidase

In prokaryotes, the protons are pumped actively across the ______ membrane by the electron transport carriers.

cellular

At best, cells can generate ATP from the complete oxidation of one glucose molecule during aerobic cellular respiration.

38

The ETS is found embedded in the inner membrane of the of eukaryotes while the ETS is found embedded in the membrane of prokaryotes.

Mitochondria; cell

How many molecules of carbon dioxide are generated by the Krebs cycle for every glucose entering glycolysis?

6

The return of nitrogenous compounds to the atmosphere at the end of the nitrogen cycle is called .

denitrification

Cytochrome aa3, also known as , catalyzes the terminal step of aerobic respiration.

cytochrome oxidase

What is the ecological benefit of denitrification?

Replenishes nitrogen in the atmosphere

Which compound blocks cytochrome oxidase, causing a termination of aerobic respiration and death?

Cyanide

Fermentation results in the production of ______ ATPs than aerobic and anaerobic respiration.

less

What is the maximum yield of ATP from aerobic respiration in prokaryotes?
Multiple choice question.

38

Eventually, carbon dioxide molecules will be produced from one molecule of glucose by the end of respiration.

6

The reduction of nitrite to nitrous oxide or nitrogen gas by some species of bacteria is called ________.

Denitrification

The incomplete breakdown of glucose in the absence of oxygen that yields only a small amount of ATP and produces a variety of byproducts is a process called .

ermentation

is an important process that is involved in the recycling the nitrogen contained in organisms back into the biosphere.

Denitrification

Aerobic respiration requires , whereas fermentation and anaerobic respiration occur without this molecule.

oxygen

Which serves as a terminal electron acceptor in fermentation?

Organic compounds

When supplies have been depleted in human muscle cells, ATP can be produced by acid fermentation for short periods of time.

oxygen; lactic

Which is NOT associated with fermentation?
Multiple choice question.

Oxygen

Which term describes the incomplete breakdown of glucose due the absence of an electron acceptor for the electron transport system?

Fermentation

Typically, ATP are netted by fermentation.

2

True or false: Fermentation uses an organic compound for the electron acceptor.

True

When would human cells use fermentation as opposed to respiration to produce ATP?

When oxygen is low

Consider the simplified aerobic respiration formula: Sugar + O2 --> ATP + CO2 + Heat ( is simply NOT part of the fermentation pathway)

oxygen

Fermentation allows survival and growth in the absence of and allows colonization of environments.

oxygen anaerobic

Which is NOT an advantage of fermentation to microbial survival and growth?
Multiple choice question.

Promotes growth in oxygen-rich areas of the host

How many net ATP are generated during fermentation?

2

What adaptation explains how some fermentative bacteria can grow as fast as they would in the presence of oxygen?

Increased rate of glycolysis

The end product of fermentation by human muscle cells is .

lactic acid

Identify two different fermentation pathways.

Alcoholic fermentation
Acidic fermentation

Some fermentative bacteria can grow as fast under anaerobic conditions as they do in aerobic conditions due to the increase in (ATP generating metabolic process) that fermentation provides.

glycolysis

Human cells can

use both aerobic respiration and fermentation pathways

True or false: Fermentation uses an organic compound for the electron acceptor.
True false question.

True

After the generation of pyruvate, pyruvate is converted into which is typically reduced to form ethanol during alcohol fermentation.

Acetaldehyde

The two general categories of fermentation pathways are and fermentation.

alcoholic or alcohol
acidic or acid

Which is typically reduced to form ethanol during fermentation?

Acetaldehyde

After glycolysis, which is the initial step in alcoholic fermentation?

Conversion of pyruvic acid to acetaldehyde

During fermentation, (NAD or NADH) is oxidized to form (NAD or NADH).

NADH
NAD

In alcohol fermentation, pyruvate is converted to acetaldehyde which is then converted to .

ethanol

Which type of acidic fermentation produces mixed acid products?

Heterolactic

Which type of fermentation best describes degradation of pyruvic acid that results in the production of some combination of lactic, acetic, succinic, and formic acids?

Mixed acid

Which occurs during fermentation?

NADH is oxidized to NAD

Which of the following is true regarding alcohol fermentation within cells?

Several different alcohols can be produced

The production of mixed acids, one being lactic acid, during fermentation is a good definition of fermentation.

Heterolactic

In mixed acid fermentation, pyruvic acid is converted into some combination of

lactic acid
acetic acid
formic acid
succinic acid

Which of the following is true regarding alcohol fermentation within cells?
Multiple choice question.

Several different alcohols can be produced

Alcoholic fermentation in bacteria could result in the production of:

...

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What type of microorganisms are able to survive and grow in refrigeration temperatures?

Unlike most bacteria, Listeria can grow and multiply in your freezer and refrigerator. This is surprising to some. Listeria can also be found in soil, water, and some animals, including poultry and cattle. It can also be present in raw milk and foods made from raw milk.

What term could be used for microbes that grow best in the refrigerator?

Psychrotrophs grow well at refrigeration temperatures, but better at room temperature. Mesophiles grow best at or near human body temperature, but grow well at room temperature. Thermophiles grow only at temperatures about as hot as the human hand can endure, and usually not at all at or below body temperature.

Which term describes microorganisms that thrive under high hydrostatic pressure?

Biofilm in High-Pressure Environments. Piezophiles (barophiles) are microorganisms whose survival and reproduction is optimized to high pressures, such as those in deep-sea environments.

Is a microorganism that thrives in a hypertonic medium?

Microorganisms that can do this and thus tolerate hypertonic environments are osmotolerant. Osmotolerant bacteria, such as Staphylococcus aureus can grow in a wide variety of environments with varying osmotic pressures.