What is the required specimen amount when collecting blood culture on an infant?

LAB TEST

COLLECTION DEVICE

AMOUNT **

(CC) WHOLE BLOOD

MICROTAINER TUBE

STANDARD TUBE

Amikacin

Gold cap with gel

0.6

Amino Acids, Plasma

Light green cap with gel

0.7

Ammonia

*Test cannot be done micro

Green Cap (3 mL), pre-chilled

2.0 On Ice

Basic Metabolic Panel

Light green cap with gel or Gold cap with gel

0.6

Bilirubin (Total & Direct)

Light green cap with gel or Gold cap with gel

0.6

Blood Culture

*Test cannot be done micro

Blood culture bottle

1.0

BUN/Creatinine

Light green cap with gel or Gold cap with gel

0.5

Calcium

Light green cap with gel or Gold cap with gel

0.6

Calcium, Ionized

*Test cannot be done micro

Gold cap

2.0

Carbamazepine

Light green cap with gel or Gold cap with gel

0.6

CBC

Lavender cap

0.5 microtainer

1.0 standard tube

Chromosome Analysis

*Test cannot be done micro

Dark green cap

2.0

Comprehensive Metabolic Panel

Light green cap with gel or Gold cap with gel

0.7

Cortisol

Gold cap with gel (2)

1.2

Digoxin

Light green cap with gel or Gold cap with gel

0.7

Dilantin

Red cap

0.6

Erythrocyte Sedimentation Rate

*Test cannot be done micro

Lavender cap

1.5

Ferritin

Light green cap with gel or Gold cap with gel

0.6

Gentamicin

Light green cap with gel or Gold cap with gel

0.6

GGT, AST, ALT

Light green cap with gel or Gold cap with gel

0.6

Glucose

Light green cap with gel or Gold cap with gel

0.6

Hematocrit

Lavender cap

1.0

Hepatic Panel

Light green cap with gel or Gold cap with gel

0.6

Hep A Antibody

Light green cap with gel or Gold cap with gel (2)

1.2

Hep B Surface Antigen

Light green cap with gel or Gold cap with gel (2)

1.2

Hep B Surface Antibody

Light green cap with gel or Gold cap with gel (2)

1.2

HyperAl Panel

Light green cap with gel or Gold cap with gel

0.6

HyperAl Panel & Theophylline

Light green cap with gel (2) or Gold cap with gel (2)

1.0-1.2

IgG

Light green cap with gel or Gold cap with gel

0.6

Lead

Lavender cap

Beige cap (preferred)

Lavender cap

0.5 microtainer

1.0 standard tube

Magnesium

Light green cap with gel or Gold cap with gel

0.6

Osmolality, Plasma

Light green cap with gel

0.7

Osmolality, Serum

Gold cap with gel

0.7

Phenobarbital

Light green cap with gel or Gold cap with gel

0.6

Phenytoin

Red cap

0.6

Phosphorus

Light green cap with gel or Gold cap with gel

0.5

Primidone

Red cap (2)

2.0

PT, PTT

*Test cannot be done micro

Light blue cap

2.7

Reticulocyte Count

Lavender cap

0.5 microtainer

1.0 standard tube

Rubella IgG

Light green cap with gel or Gold cap with gel (2)

1.2

Sodium, Potassium

Light green cap with gel or Gold cap with gel

0.5

Sodium, Potassium, Bilirubin

Light green cap with gel or Gold cap with gel

0.7

Sodium, Potassium, Calcium

Light green cap with gel or Gold cap with gel

0.6

Syphilis Screen

Gold cap with gel (2)

1.0

T3

Light green cap with gel or Gold cap with gel (2)

1.2

T4, Free

Light green cap with gel or Gold cap with gel

0.6

T4, Total

Light green cap with gel or Gold cap with gel

0.6

Tegretol

Light green cap with gel or Gold cap with gel

0.6

Theophylline

Light green cap with gel or Gold cap with gel

0.6

Tobramycin

Light green cap with gel or Gold cap with gel

0.6

Triglyceride

Light green cap with gel or Gold cap with gel

0.6

TSH

Light green cap with gel or Gold cap with gel

0.6

Type and Crossmatch

Lavender cap (neonates only) 

Pink cap

0.5 microtainer

2.0 standard tube

Valproic Acid

Light green cap with gel or Gold cap with gel

0.6

Vancomycin

Light green cap with gel or Gold cap with gel

0.6

*When we state that Laboratory Alliance cannot use a microtainer or finger/heel stick blood, the reason is not because of the quantity of blood.  Some lab tests cannot use capillary blood because there are slight differences in the composition of capillary blood vs. venous blood.  These differences would interfere with the test methodology and would cause inaccuracies if performed with capillary blood.

**Neonates with high HCT’s will need more blood than listed

3/2017

What is the required specimen amount when collecting a blood culture from an infant?

The standard method is to collect at least 5 to 10 ml blood per culture bottle from patients with fevers.

How much blood can you draw from an infant?

According to research on safe blood draws in infants, it is recommended by several studies and guidelines that the maximum amount of blood drawn from infants and children in 24 hours be between 1% and 5% of the total blood volume (TBV) of the child. Ideally, the amount should be less than 3% of the TBV.

How is blood drawn from a 9 month old?

In babies, blood draws are sometimes done as a "heel stick collection." After cleaning the area, the health professional will prick your baby's heel with a tiny needle (or lancet) to collect a small sample of blood. Collecting a sample of blood is only temporarily uncomfortable and can feel like a quick pinprick.

What is the preferred method of collecting blood from infants and neonates?

Blood collection from the heel is the standard for newborn screening. The medial and lateral parts of the underfoot are preferred.